1 How to order peptides at Bioyeargene?

Pleasesend the specific requests(sequence, purity, amount, etc.) to email address bioyeargene@163.com or bioyears@hotmail.com, we will respond intime with quotes and solutions.

2 What is the usual turnaround time for peptide synthesis at Bioyeargene?

Usualturnaround time is 1-2 weeks. It may vary depending on the peptide length andcomplexity.

3 What data will be provided?

Foreach peptide, QC reports containing the amino acid sequence, modificationinformation, peptide purity, mass spectrum, and HPLC chromatogram will beprovided.

4 What if there is a problem with the synthesis of my peptide?

Eachpeptide has specific characteristics and the outcome of a synthesis attemptcannot always be anticipated. If for some reason we cannot deliver your peptideon time, we will inform you as soon as possible. However, we will always tryour best even for 3-4 times to make it.

5 When should I make payment for peptides? What kinds of payment do you accept?

Customerscan make payment within one month upon receipt after checking the received peptidesto confirm it meets the requirements. We can receive payments through ourcompany’s account or Paypal account.

6 What if I find that there is a problem with the received peptides?

Usuallywe strictly synthesize peptides according to customers’ requirements insequence, purity and amount. As for the activity of peptides, we can’t makesure that it definitely takes good effect in customer’s research, however, forhistorical peptides that we made, we can try our best to make high batch-tobatch consistency.

7 How should I store my peptides?

Lyophilizedpeptides can be stored long-term at -20°C.

8 What is the best way to dissolve my peptide?

Thesolubility of a given peptide varies depending on its amino acid sequence andmodifications. Bioyeargene purifies peptides by HPLC using a water andacetonitrile gradient. Below are some general tips for dissolving peptides:

Sonicationwill increase solubility.

Add10% acetic acid to your solvent to help dissolve basic peptides.

Add10% ammonium bicarbonate to your solvent to help dissolve acidic peptides.

Forpeptides with extremely low solubility in aqueous solutions, try adding organicsolvents (such as DMSO, isopropanol, methanol, or acetonitrile) first. Once thepeptides are completely dissolved, water may be gradually added until the desiredconcentration is obtained.

9 What is net peptide content?

Itis important to understand the difference between net peptide content and totalpeptide content. The dry peptide powder shipped to you usually contains notonly peptide, but also some other substances such as water, absorbed solvents,counter ions, and salts. The total peptide content refers to the weight of thismixture. Net peptide weight indicates the actual weight of the peptidecomponent of your sample. Net peptide content is usually 50-80% of the totalpeptide weight (also called gross peptide weight) and is usually determined byamino acid analysis or N element analysis. Net peptide content should not beconfused with purity. Purity defines the percentage of the target peptidesequence in the peptide component of your sample.

10 How do you calculate theoretical net peptide content?

Theoreticalnet peptide content (calculated assuming that counterions are the onlynon-peptide components present in your peptide sample) can be estimated bydividing the molecular weight of the peptide by a sum of the molecular weightand a number of trifluoroacetate counterions that are required to neutralizethe peptide. The sum is multiplied by the molecular weight of the TFAcounterion (MW= 114). For example, a synthetic peptide of MW=1000 with a freeN-terminal amino group and one Arg has theoretical net peptide content of1000/(1000 + 2 x 114 ) = 1000/1228 =0.81 or 81%. In practice, counterions arenot the only possible contaminants in the peptide sample. Peptide samples canalso contain water, absorbed solvents and traces of other substances. As aresult, the actual net peptide content is usually determined by quantitativeamino acid analysis.